CHO Edge System
A state-of-the-art biologics expression system that routinely achieves 8-12 g/L across modalities before process development
Cell line development has always involved a lot of guesswork. CHO Edge replaces that with data-driven engineering.
Our system covers every stage from discovery through GMP manufacturing, combining computational design with a high-performance CHO host and vector design tools. The goal: predictable, high-titer results with fewer surprises at scale.
An end-to-end solution for biologics developers
We offer integrated solutions for your biologics expression needs, from lead optimization, to CLD, to process & analytical development:
The CHO Edge System
Rapid Pools
Transient expression systems often don't predict how a molecule will actually perform when manufactured at scale using stable cell lines. That can mean selecting leads based on data that won't hold up later.
Rapid Pools addresses this by using the same CHO host, vectors, and media from our stable CLD process to generate characterized pools in two weeks. You get manufacturability data early, which helps you identify problematic candidates before they cost you time and money.
Cell Line Development
CHO Edge routinely produces clones at 8-12 g/L before process development across modalities: standard mAbs, fusion proteins, 2-chain, 3-chain, and 4-chain bispecifics.
We use our Kernel design software to optimize vector variants for your specific molecule, whatever its architecture. The result is a high-titer Research Cell Bank delivered in 14 weeks guaranteed.
Process Development
A great clone means nothing if it doesn't perform at scale.
We pair our CHO Edge host with hybrid physics-informed machine learning models to co-optimize the clone and process together. This approach is designed to support consistent performance from screening through 2,000L GMP production.
Ready to talk?
Rapid Pools
Transient expression systems often don't predict how a molecule will actually perform when manufactured at scale using stable cell lines. That can mean selecting leads based on data that won't hold up later.
Rapid Pools addresses this by using the same CHO host, vectors, and transposase from our stable CLD process to generate characterized pools in two weeks. You get manufacturability data early, which helps you identify problematic candidates before they cost you time and money.
Rapid Pools offers predictive CLD behavior with an option to go all the way to RCB with Asimov's CHO Edge Cell Line Development Services.
Benefits
Derisk lead selection
- Prove out molecules in a CLD-like context, minimizing late-stage failures and suboptimal performance.
Faster time-to-market
- Avoid timeline-disrupting churn; gain the benefits of stable integration on short timelines.
Reduce costs
- Avoid use of costly transient production consumables, unlock higher yields and reproducible scale-up.
Bridging the gap between lead optimization and CLD
Predictive discovery-to-CMC translation
While traditional transient workflows require costly re-engineering to switch from discovery to manufacturing, Asimov Rapid Pools deliver stable, predictive CHO material early in the lead optimization phase, allowing you to select developable candidates immediately and scale up without delay. The product quality and titer data you see in discovery reliably predicts future GMP performance, effectively derisking candidate selection.
System components
Robust host cell lines
We developed a glutamine synthetase (GS) knock-out cell line for producing antibodies, multispecifics, and other protein modalities.
To enable glycan engineering, we also generated a GS/fucosyltransferase (FUT8) double knock-out line that yields 100% afucosylated antibodies with a 10-fold boost in antibody-dependent cellular cytotoxicity (ADCC).
Rapid hyperactive transposase and genetic parts library
We have developed a hyperactive transposase that enables stable expression with rapid recovery and a library of >1000 characterized genetic parts for use in CHO cell line development. These genetic parts span different cell functions, including promoters, untranslated regions, epigenetic insulators, signal peptides, polyadenylation signals, inducible systems, genome editors, and more.
High-throughput CLD-grade workflows
We have engineered a high-throughput, CLD-grade workflow that utilizes lab automation and miniaturization to generate hundreds of stable pools in just 10 weeks. From DNA design and transfection through bulk pool recovery, our integrated process ensures rapid initial screening and robust expression. This platform delivers a reusable, scalable, and reproducible manufacturing solution that is validated by comprehensive purification and analytical panels.
Data-driven vector design with Kernel
Asimov's computer-aided design (CAD) software, Kernel, can be used to optimize vector designs. It achieves this through the use of our proprietary biophysical/AI models of transcription, translation, secretion, and more.
Ready to derisk your lead selection?
Cell Line Development
CHO Edge Cell Line Development routinely produces clones at 8-12 g/L across modalities: standard mAbs, fusion proteins, 2-chain, 3-chain, and 4-chain bispecifics.
We use our Kernel design software to optimize vector variants for your specific molecule, whatever its architecture. The result is a high-titer Research Cell Bank delivered in 14 weeks guaranteed.
How to work with us
We provide tools for you to develop a cell line
We will ship the software, cells, and genes you need to design your cell line in-house.
We develop a cell line for you
Using our platform, we will design a custom cell line specifically for your needs and ship it to you.
We provide tools for you to develop a cell line
We will ship the software, cells, and genes you need to design your cell line in-house.
You design DNA
You use our software to design and optimize genetic constructs using best-in-class parts and models.
We build DNA
We will ship the software, cells, and genes you need to design your cell line in-house.
You transfect cells
You transfect and generate stable cell lines and use our software for data analysis.
You request producer cells
You request a producer cell line for a biologic or gene therapy.
We do cell line development
We do cell line development and process development at our site.
We share progress
We share progress and data with you using our software.
We ship you producer cell banks
We ship you clonal cell banks and transfer the process to your site.
We provide tools for you to develop a cell line
We will ship the software, cells, and genes you need to design your cell line in-house.
We develop a cell line for you
Using our platform, we will design a custom cell line specifically for your needs and ship it to you.
We provide tools for you to develop a cell line
We will ship the software, cells, and genes you need to design your cell line in-house.
You design DNA
You use our software to design and optimize genetic constructs using best-in-class parts and models.
We build DNA
We will ship the software, cells, and genes you need to design your cell line in-house.
You transfect cells
You transfect and generate stable cell lines and use our software for data analysis.
You request producer cells
You request a producer cell line for a biologic or gene therapy.
We do cell line development
We do cell line development and process development at our site.
We share progress
We share progress and data with you using our software.
We ship you producer cell banks
We ship you clonal cell banks and transfer theprocess to your site.
We develop a cell line for you
Using our platform, we will design a custom cell line specifically for your needs and ship it to you.
We provide tools for you to develop a cell line
We will ship the software, cells, and genes you need to design your cell line in-house.
You design DNA
You use our software to design and optimize genetic constructs using best-in-class parts and models.
We build DNA
We will ship the software, cells, and genes you need to design your cell line in-house.
You transfect cells
You transfect and generate stable cell lines and use our software for data analysis.
You request producer cells
You request a producer cell line for a biologic or gene therapy.
We do cell line development
We do cell line development and process development at our site.
We share progress
We share progress and data with you using our software.
We ship you producer cell banks
We ship you clonal cell banks and transfer the process to your site.
Two ways to work with us
Whether you need a turnkey solution delivered by our experts or the tools to build it yourself, we offer two ways to access the CHO Edge System.
Full CLD services done in our lab
Leverage Asimov’s state-of-the-art Boston facility and expert team to handle your campaign from start to finish. We deploy our full technology stack to deliver a high-titer Research Cell Bank (RCB) in just 14 weeks.
Best for partners seeking industry-leading speed, reduced operational risk, and performance guarantees (5 g/L or it's free for mAbs).
We perform cell line development for your molecules
Your cell line development campaign begins with your amino acid sequence. CLD is done at our site using state-of-the-art equipment with industry-leading timelines.
We ship you clonal, stable cell lines
Vials of research cell banks are shipped to your lab, the process is transferred to your site, and documentation for your regulatory filing is supplied.
Optional add-on services and support
Early-look material generated in benchtop bioreactors, stability study on top clones, upstream process optimization, master cell banking coordination, and tech transfer support into GMP environments.
System licensing & technology transfer
Equip your own laboratory with Asimov’s complete ecosystem. Under this model, we transfer our proprietary materials and software directly to your team, enabling you to run the CHO Edge System at your own site.
Best for partners who want to integrate our capabilities into their existing infrastructure or maintain internal control over the entire development lifecycle.
We ship you CHO cells and grant software access
Vials of GMP-banked CHO cells with regulatory documentation are shipped to you, and your team is onboarded into our plasmid design software, Kernel.
You design plasmids for your molecules
Using our parts and computational models, you design a set of expression plasmids. We’re here to help if needed. Optionally, we can build and ship you plasmids.
You perform cell line development
You transfect and generate stable cell lines, with technical support from our team.
We provide tools for you to develop a cell line
We will ship the software, cells, and genes you need to design your cell line in-house.
We develop a cell line for you
Using our platform, we will design a custom cell line specifically for your needs and ship it to you.
We provide tools for you to develop a cell line
We will ship the software, cells, and genes you need to design your cell line in-house.
You design DNA
You use our software to design and optimize genetic constructs using best-in-class parts and models.
We build DNA
We will ship the software, cells, and genes you need to design your cell line in-house.
You transfect cells
You transfect and generate stable cell lines and use our software for data analysis.
You request producer cells
You request a producer cell line for a biologic or gene therapy.
We do cell line development
We do cell line development and process development at our site.
We share progress
We share progress and data with you using our software.
We ship you producer cell banks
We ship you clonal cell banks and transfer the process to your site.
Full CLD services done in our lab
Leverage Asimov’s state-of-the-art Boston facility and expert team to handle your campaign from start to finish. We deploy our full technology stack to deliver a high-titer Research Cell Bank (RCB) in just 14 weeks.
Best for partners seeking industry-leading speed, reduced operational risk, and performance guarantees (5 g/L or it's free for mAbs).
We provide tools for you to develop a cell line
We will ship the software, cells, and genes you need to design your cell line in-house.
You design DNA
You use our software to design and optimize genetic constructs using best-in-class parts and models.
We build DNA
We will ship the software, cells, and genes you need to design your cell line in-house.
You transfect cells
You transfect and generate stable cell lines and use our software for data analysis.
We perform cell line development for your molecules
We ship you clonal, stable cell lines
Optional add-on services and support
System licensing & technology transfer
Equip your own laboratory with Asimov’s complete ecosystem. Under this model, we transfer our proprietary materials and software directly to your team, enabling you to run the CHO Edge System at your own site.
Best for partners who want to integrate our capabilities into their existing infrastructure or maintain internal control over the entire development lifecycle.
We develop a cell line for you
Using our platform, we will design a custom cell line specifically for your needs and ship it to you.
We ship you host cells and grant software access
You design plasmids for your molecules
You perform cell line development
You request producer cells
You request a producer cell line for a biologic or gene therapy.
We do cell line development
We do cell line development and process development at our site.
We share progress
We share progress and data with you using our software.
We ship you producer cell banks
We ship you clonal cell banks and transfer theprocess to your site.
Benefits of CLD services
Reduce the risk of low titer
- With our CHO Edge System, titers are typically 8-12 g/L or higher for both IgG monoclonal antibodies and more complex bispecific and trispecific architectures.
5 g/L or it's free
- We guarantee high titer cell lines for monoclonal antibodies. If the RCB generated for a mAb is under 5 g/L, the CLD campaign and all commercial use rights are free.
Regulator approved
- IND approved system and a team with decades of experience in CLD and product commercialization.
How it works
From amino acid sequence to high-titer Research Cell Bank in 14 weeks.
Computational design
We use our proprietary software, Kernel, for codon optimization, vector design, and biophysical simulation to make a panel of expression vectors.
You request a cell line development campaign for a specific biologic and provide us with an amino acid sequence.
CLD start
GS-KO CHO host transfected
Expression vectors are transfected to generate a panel of stable pools which are assessed for quality and titer performance.
3 weeks
Stable pool generated
The lead pool is cloned out with monoclonality verification via pre and post deposition imaging using the Cytena F-sightTM and Solentim Cell Metric®.
8 weeks
Clones characterized and banked
24-48 clones are progressed for fed-batch analysis at Ambr® 15 scale.
12 weeks
Bioreactor Ambr® 15 run
Additional process development using Ambr® 250 and bench top bioreactors is also available.
Stability studies and additional analytical characterization can be performed at your request.
14 weeks
Research cell bank release
We ship the top clones as RCBs to you or your preferred GMP manufacturing partner.
What you get
Top 5 clones
- Validated high-performers producing your specific therapeutic protein of interest
>80% titer stability
- Transposase-mediated integration ensures clones maintain stability across 75 generations
CMC-compliant documentation
- Full lineage and transposon integrity data to support your IND filing
Prefer to transfer the CHO Edge System to your own lab?
Chat to a member of our team about Asimov developing your next cell line
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